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Scibet Review
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- Furthermore, each cell type could be further classified into more precise labels based on datasets uncovering novel sub cell types.
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We curated the cell label by unifying the major cell type annotated by different publications (e.g., considering both “T lymphoid cells” and “T cells” as “T cells”). We neglected the minor cell types, which means those novel subtypes uncovered by scRNA-seq with unsupervised clustering and annotation (e.g., considering both “DC_cluster1” and “DC_cluster2” as DC cells). For cross-validation benchmarks, we performed the classification using the function scmapCluster of scmap without considering the unassigned cells.
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In addition, SciBet balances high accuracy and low false positive rate by setting a null dataset as the alternate reference for cells with types not yet covered by the existing data. Finally, we provide both local and web-based SciBet implementations that are compatible with either existing or custom datasets for ultra-fast and accurate cell type identification. SciBet addresses an important need in the rapidly evolving field of single-cell transcriptomics, i.e., to accurately and rapidly capture main features of diverse datasets regardless of technical factors or batch effect. Based on multiple benchmarks, SciBet achieves high prediction accuracy, while keeping low false positive rate for cells not represented previously.
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